Optimasi Ekstraksi DNA Pseudonocardia Carboxydivorans 18A213O1 Menggunakan Kit Ekstraksi Promega dan Qiagen
DOI:
https://doi.org/10.31599/js4jb268Keywords:
: Tunicate, Pseudonocardia carboxydivorans, Promega extraction kit, Qiagen extraction kitAbstract
More than 70% of the earth's oceans are known to cover the surface of the earth. High biodiversity makes the ocean a habitat for microorganisms such as tunicates. Tunicates can associate with other microorganisms such as Pseudonocardia carboxydivorans so that they can produce bioactive compounds including antibiotics, pesticides and antitumors. The initial stage to identify a bioactive compound is by isolating DNA. This study aims to determine the optimization of bacterial DNA extraction using promega and qiagen extraction kits. This study consists of 3 treatments, namely rejuvenation, bacterial identification and concentration measurement. The results showed that the optimization of using qiagen extraction kit was relatively superior at 1,989 ng/µl - 2,000 ng/µl with 1 hour. While in the optimization of promega extraction, the resulting purity is 1,500 ng/µl - 1,943 ng/µl with a processing time of 2 hours.
Downloads
References
Bellard, M. G., Pierre, O., & Pierre, C. (1973). Isolation of High-Molecular-Weight DNA from MamalianCells. Europian Journal of Biochemistry, 36(1): 32-38. https://doi.org/10.1111/j.1432-1033.1973.tb02881.x.
Diah, A., Rhesi, K., & Mezan, A. (2020). Potensi Bakteri Asosiasi Tunikata Sebagai Penghasil Senyawa Antibakteri Guna Menghambat Pertumbuhan Bakteri Multidrug Resistent. Pasir Laut, 4(2): 102-107. https://doi.org/10.14710/jpl.2020.32807.
Disha, V., Jiun, Y. W., Khatijah, Y., Rabiha, S., Swee, H., Kok, S., & Chou, M. (2021). Bioactive Compounds from Marine Sponges: Fundamentals and Applications. Marine drugs, 19(5): 246. https://doi.org/10.3390%2Fmd19050246.
Emilia, Essy, H., & Ashabul, A. (2021). Optimasi Metode Ekstraksi DNA Daun, Kulit Kayu dan Kayu Pinus Merkussi Jungh. Et De Vriese. Ilmiah Mahasiswa Pertanian, 6(4): 766-778. https://jim.usk.ac.id/JFP/article/view/18233 .
Hasrida, M. I., & Yusran, U. (2016). Pengukuran Konsentrasi dan Kemurnian DNA Genom Nyamuk Anopheles barbirotris. Vektor Penyakit, 10(1). 7-9. DOI:10.22435/vektorp.v10i1.6251.7-10.
Huang, G. P., Jie, Y., Li, S., Zhi, G. S., Jue, H. W., Xiu, J. L., . . . Sheng, P. C. (2011). Statistical Research on Marine Natural Products Based on Data Obtainded Between 1985 and 2008. Marine Drug, 9(4): 514-525. https://doi.org/10.3390%2Fmd9040514.
Mark, J., & Chhaya, C. (2017). Marine Biodiversity, Biogeography, Deep-Sea Gradients and Conservation. Current Biology, 27(11): 511-527. http://dx.doi.org/10.1016/j.cub.2017.04.060.
Promega. (2023, October Wednesday). Wizard Genomic DNA Purification Kit. Retrieved from www. promega.com: https://www.promega.com/products/nucleic-acid-extraction/genomic-dna/wizard-genomic-dna-purification-kit/
Qiagen. (2023, October Wednesday). QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook. Retrieved from www.qiagen.com: https://www.qiagen.com/jp/resources/download.aspx?id=62a200d6-faf4-469b-b50f-2b59cf738962&lang=en
Sambrook, J. E., Fritsch., & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 3rd Edition. Spring Harbor: Cold Spring Harbor.
Shanti, R., Pamella, A., Fahrurozi, Puspita, L., & Wien, K. (2016). Aktivitas Antibakteri Aktinomisetes Laut Dari Pulau Enggano. Ilmu-Ilmu Hayati, 15(3): 275-283. http://dx.doi.org/10.14203/beritabiologi.v15i3.2258.